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27/03/2022Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera
List of authors
SurachaiTechaoeiabChariyaJirayuthcharoenkulaKhemjiraJarmkomabThisakornDumrongphuttidechaabWarachateKhobjaic
- a Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi 12130 Thailand
- b Innovation of Plants Extraction and Biological Activity for Pharmaceutical Products, Food Supplement and Medical Drugs, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi 12130 Thailand
- c Department of Clinical Chemistry, Faculty of Medical Technology, Nation University No. 444 Vajiravuth Damnern Road, Prabat Sub-district, Mueang District, Lampang 52000, Thailand
Abstract
The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera. All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of N. nucifera. Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as Aspergillus cejpii. Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C11H11N5). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen. View Full-text
* Author to whom correspondence should be addressed.
Techaoei, S., Jirayuthcharoenkul, C., Jarmkom, K., Dumrongphuttidecha, T., & Khobjai, W. (2020). Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera. Saudi Journal of Biological Sciences, 27(11), 2883–2889. https://doi.org/10.1016/j.sjbs.2020.08.037
